Establishment of chromium detecting kit and its application in sea water and seafood

Chromium is one of main pollution in sea water, and the establishment detecting kit research hot topic. In this study, immunized mice were obtained by immunogen Cr 3+ -EDTA-BSA, hybridoma strains that secrete -EDTA monoclonal antibody selected. mAb prepared with vivo method. Then, Cr-kit for measurement chromium was developed, its application executed water seafood. Two A321 A424 screened. The standard curve linear range 1.0—512 μg/L, limit detection 1.0 μg/L. average recovery rates spiked samples clam shrimp 102.62%, 94.69% 96.29%, which accordance results ICP-AES. Totally, successfully developed behaved broad potential